Uji Aktivitas Antibakteri Air Perasan Jeruk Siam Sambas terhadap Escherichia coli
DOI:
https://doi.org/10.62383/vimed.v2i1.1021Keywords:
antibacterial, citrus siam sambas, Escherichia coli, juice extract, phytochemical screeningAbstract
Escherichia coli is a bacterium that typically resides as a normal flora in the gastrointestinal tract of humans and animals. However, certain strains of E. coli can cause infections in humans. Therefore, antibiotics are needed to inhibit the growth of these infection-causing bacteria. The increasing cases of antibiotic resistance in E. coli necessitate alternative therapies, including the use of natural substances. In West Kalimantan, one potential source is the Pontianak Orange (Citrus nobilis var. microcarpa). This plant contains secondary metabolites that can be used for medicinal purposes, such as the juice extract. The compounds within these secondary metabolites possess antimicrobial properties that could be used as antibacterials. To determine the antibacterial potential of Pontianak Orange juice (Citrus nobilis var. microcarpa) against Escherichia coli. This study utilized an experimental design featuring a post-test only group design. Phytochemical analysis indicated that Pontianak Orange juice (Citrus nobilis var. microcarpa) contains phenolics (+), alkaloids (+), and terpenoids (++). Antibacterial activity testing at all concentrations (25%, 50%, 75%, and 100%) showed no inhibition zones. The juice of Pontianak Orange (Citrus nobilis var. microcarpa) did not show antibacterial effects against the growth of Escherichia coli bacteria.
References
Anisa, G., Juliansyah, E., & Sohibun, S. (2023). Faktor-Faktor Yang Berhubungan Dengan Kejadian Diare Pada Balita Di Wilayah Kerja Puskesmas Nanga Pinoh Kabupaten Melawi Tahun 2022. Jurnal Ilmiah Kesehatan Masyarakat. .
Apriliani, M., Ramadhan, A. M., Rijai, L., Penelitian, L., Pengembangan, D., & Tropis, F. (2017). Aktivitas Antibakteri Ekstrak Daun Jeruk Sambal (Citrus Microcarpa) Terhadap Beberapa Bakteri Patogen.
Berlian, Z., Fatiqin, A., & agustina, eka. (2016). Penggunaan Perasan Jeruk Nipis (Citrus aurantifolia) Dalam Menghambat Bakteri Escherichia coli Pada Bahan Pangan. Bioilmi: Jurnal Pendidikan, 2(1), 51. https://doi.org/10.19109/BIOILMI.V2I1.1139
Bouarab-Chibane, L., Forquet, V., Lantéri, P., Clément, Y., Léonard-Akkari, L., Oulahal, N., … Bordes, C. (2019). Antibacterial properties of polyphenols: Characterization and QSAR (Quantitative structure-activity relationship) models. Frontiers in Microbiology, 10(APR), 440698. https://doi.org/10.3389/FMICB.2019.00829/BIBTEX
Cheong, M. W., Zhu, D., Sng, J., Liu, S. Q., Zhou, W., Curran, P., & Yu, B. (2012). Characterisation of calamansi (Citrus microcarpa). Part II: volatiles, physicochemical properties and non-volatiles in the juice. Food Chemistry, 134(2), 696–703. https://doi.org/10.1016/J.FOODCHEM.2012.02.139
Endang Christine Purba, & Bambang S. Purwoko. (2019). Teknik Pembibitan, Pemupukan, Dan Pengendalian Hama Penyakit Tanaman Komoditi Jeruk Siam (Citrus nobilis Var. Microcarpa) Di Kecamatan Simpang Empat Dan Kecamatan Payung, Kabupaten Karo, Sumatra Utara, Indonesia. Jurnal Pro-Life, 6.
Hudzicki, J. (2009). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. Retrieved from www.atcc.org
Jawetz, Melnick, & Adelberg. (2022). Mikrobiologi Kedokteran.
Jessica Magdalena Kambey, B., Sudewi, S., & Jayanto, I. (2019). Analisis Korelasi Antara Kandungan Fenol Total Dengan Aktivitas Antibakteri Ekstrak Dan Fraksi Abelmoschus manihot L. Terhadap Escherichia coli. PHARMACON, 8(2), 472–479. https://doi.org/10.35799/PHA.8.2019.29315
Katzung, B. G., Masters, S. B., & Trevor, A. J. (2023). Basic & Clinical Pharmacology. Retrieved from http://www.usdoj.gov/dea/pubs/scheduling.html
Kementrian Kesehatan Republik Indonesia. (2020). Keputusan Direktur Pengendalian Penyakit Tidak Menular Pengendalian Penyakit Dan Penyehatan Lingkungan Nomor : Hk. 02.03/6/ 1391/2020. Rencana Aksi Kegiatan Direktorat Pengendalian Penyakit Tidak Menular Tahun 2020-2024.
Mardhia, Liana, D. F., Mahyarudin, & Hariyanto. (2022). Pedoman Antibiotik Empiris (Vol. 2nd).
Mutu, P., Jeruk, B., Berdasarkan, S., Handayani, N., Akhiriani, S., & Rahmawati, A. (2023). Peningkatan Mutu Buah Jeruk Siem Berdasarkan Masa Panen Basis Kalender dan Karakteristik Fisik dan Sensorisnya. Jurnal Agroteknologi, 17(02), 83–93. https://doi.org/10.19184/J-AGT.V17I02.43096
Nurulita, Y., Yuharmen, Y., Nenci, N., Mellani, A. O., & Nugroho, T. T. (2020). Metabolit Sekunder Sekresi Jamur Penicillium spp. Isolat Tanah Gambut Riau sebagai Antijamur Candida albicans. Chimica et Natura Acta, 8(3), 133. https://doi.org/10.24198/CNA.V8.N3.32452
Oikeh, E. I., Omoregie, E. S., Oviasogie, F. E., & Oriakhi, K. (2016). Phytochemical, antimicrobial, and antioxidant activities of different citrus juice concentrates. Food Science & Nutrition, 4(1), 103. https://doi.org/10.1002/FSN3.268
Poirel, L., Madec, J.-Y., Lupo, A., Schink, A.-K., Kieffer, N., Nordmann, P., & Schwarz, S. (2018). Antimicrobial Resistance in Escherichia coli. Microbiology Spectrum, 6(4). https://doi.org/10.1128/MICROBIOLSPEC.ARBA-0026-2017
Pracaya; (2002). Jeruk Manis, Varietas, Budidaya Dan Pascapanen. Penebar Swadaya. Retrieved from //perpus.uir.ac.id%2Findex.php%3Fp%3Dshow_detail%26id%3D47448%26keywords%3D
Purwanti, F., Trimulyono, G., Biologi, J., Matematika, F., Pengetahuan, I., Universitas, A., & Surabaya, N. (n.d.). Efektivitas Antibakteri Ekstrak Lichen Parmelia Sulcata terhadap Pertumbuhan Bakteri Shigella dysenteriae dan Bacillus cereus Antibacterial Effectivivess of Extract of Lichen Parmelia sulcata on the Growth of Bacterial Shigella dysenteriae and Bacillus cereus. Retrieved from http://ejournal.unesa.ac.id/index.php/lenterabio
Ramli, S., Radu, S., Shaari, K., & Rukayadi, Y. (2017). Antibacterial Activity of Ethanolic Extract of Syzygium polyanthum L. (Salam) Leaves against Foodborne Pathogens and Application as Food Sanitizer. BioMed Research International, 2017(1), 9024246. https://doi.org/10.1155/2017/9024246
Rosmarkam, A., & Yuwono, N. W. (2002). Ilmu kesuburan tanah.
Sepriani, O., Handayani, D., Kimia, P., Pmipa Fkip, J., & Bengkulu, U. (2020). Potensi Ekstrak Tumbuhan Andaliman (Zanthoxylum acanthopodium Dc.) Sebagai Antibakteri Staphylococcus Aureus Potensi Ekstrak Tumbuhan Andaliman (Zanthoxylum acanthopodium Dc.) Sebagai Antibakteri Staphylococcus aureus. ALOTROP, 4(2), 133–139. https://doi.org/10.33369/ATP.V4I2.13864
Shi, Y. bin, Liu, L., Shao, W., Wei, T., & Lin, G. mei. (2015). Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids. Journal of Zhejiang University. Science. B, 16(8), 690. https://doi.org/10.1631/JZUS.B1500121
Sholekah, F. F. (2017). Perbedaan Ketinggian Tempat Terhadap Kandungan Flavonoid Dan Beta Karoten Buah Karika ( Carica pubescens ) Daerah Dieng Wonosobo.
Sumampouw, O. J. (2018). Uji Sensitivitas Antibiotik Terhadap Bakteri Escherichia coli Penyebab Diare Balita Di Kota Manado. Journal of Current Pharmaceutical Sciences, 2(1).
Suzana Devi, & Mayang Sari Umar. (2019). Uji Aktivitas Antibakteri Air Perasan Jeruk Siam (Citrus nobilis, L) Terhadap Pertumbuhan Bakteri Eschericia coli. Jurnal Akademi Farmasi Prayoga.
Tjandra, R. F., Fatimawali, ., & Datu, O. S. (2020). Analisis Senyawa Alkaloid dan Uji Daya Hambat Ekstrak Buah Sirih (Piper betle L) terhadap Bakteri Staphylococcus epidermidis. EBiomedik, 8(2), 173–179. https://doi.org/10.35790/EBM.V8I2.28963
Vanesa, V., Asseggaf, S. N. Y. R. S., Mardhia, M., & Mahyarudin, M. (2024). Uji Aktivitas Antibakteri Air Perasan Jeruk Sambal (Citrus Microcarpa bunge) Terhadap Pertumbuhan Bakteri Staphylococcus aureus. E-Jurnal Medika Udayana, 13(5), 99. https://doi.org/10.24843/MU.2024.V13.I05.P16
Yunus, F. T., Suwondo, A., & Martini. (2021). Phytochemical Compound of Garlic (Allium sativum) as an Antibacterial to Staphylococcus aureus Growth. IOP Conference Series: Materials Science and Engineering, 1053(1), 012041. https://doi.org/10.1088/1757-899X/1053/1/012041
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