Deteksi Molekuler Tilapia Lake Virus (TiLV) pada Ikan Nila (Oreochromis niloticus) di Provinsi Kalimantan Timur, Indonesia
DOI:
https://doi.org/10.62383/ikg.v3i1.2756Keywords:
East Kalimantan, Molecular Detection, PCR, Tilapia Lake Virus, TilapiaAbstract
Tilapia Lake Virus (TiLV) is an RNA pathogen that causes high mortality rates in tilapia (Oreochromis niloticus), posing a major threat to global aquaculture. This study aimed to detect the presence of TiLV in tilapia populations in East Kalimantan Province, Indonesia, using the Polymerase Chain Reaction (PCR) method. A total of 60 tilapia samples were collected from three main aquaculture areas: Kutai Kartanegara, Samarinda, and Penajam Paser Utara. Molecular analysis revealed that one sample from Penajam Paser Utara was positive for TiLV, with an overall prevalence of 5%, while all samples from other sites tested negative. This finding indicates that TiLV has been molecularly detected in East Kalimantan, albeit at a low prevalence. The overall prevalence of TiLV infection in East Kalimantan was 1.67%. The results provide important baseline data for strengthening disease surveillance, biosecurity implementation, and the development of preventive strategies in tilapia aquaculture systems in Indonesia.
References
Akter, M. S., Rahman, M. M., Hossain, M. A., & Rahman, M. A. (2025). Investigation of tilapia mortality associated with TiLV in Bangladesh. Veterinary Medicine and Science, 11(2), 105–113. https://doi.org/xx.xxxx/vms.2025.xxx
Budi, K. L. S., Fitriyana, F., & Haqiqiansyah, G. (2004). Strategi adaptasi pembudidaya keramba jaring apung di kolam bekas tambang di Desa Bangun Rejo Kecamatan Tenggarong Seberang. Zoologi: Jurnal Ilmu Peternakan, Ilmu Perikanan, dan Ilmu Kedokteran Hewan, 1(2), 1–13. https://doi.org/10.62951/zoologi.v2i2.32
Dita, S. R., Laela, N. F., Putri, D. W. S., & Rahardjo, A. (2023). Detection of Tilapia Lake Virus (TiLV) using semi-nested RT-PCR in East Java, Indonesia. IOP Conference Series: Earth and Environmental Science, 1273(1), 012034. https://doi.org/10.1088/1755-1315/1273/1/012034
Eyngor, M., Zamostiano, R., Tsofack, J. E. K., Berkowitz, A., Bercovier, H., Tinman, S., Lev, M., Hurvitz, A., Galeotti, M., Bacharach, E., & Eldar, A. (2014). Identification of a novel RNA virus causing mass mortality in tilapia. mBio, 5(5), e00929–14. https://doi.org/10.1128/mBio.00929-14
Hardi, E. H., Nugroho, R. A., Saptiani, G., Sarinah, R., Agriandini, M., & Mawardi, M. (2018). Identification of potentially pathogenic bacteria from tilapia (Oreochromis niloticus) and channel catfish (Clarias batrachus) culture in Samarinda, East Kalimantan, Indonesia. Biodiversitas, 19(2), 480–488. https://doi.org/10.13057/biodiv/d190215
Hardi, E. H., Pebrianto, C. A., Hidayanti, T., & Handayani, R. T. (2014). Infeksi Aeromonas hydrophila melalui jalur yang berbeda pada ikan nila (Oreochromis niloticus) di Loa Kulu Kutai Kartanegara Kalimantan Timur. Jurnal Kedokteran Hewan, 8(2), 130–133.
Hardi, E. H., Sukenda, Haris, E., & Lusiastuti, A. M. (2013). Kandidat vaksin potensial Streptococcus agalactiae untuk pencegahan penyakit streptococcosis pada ikan nila (Oreochromis niloticus). Jurnal Veteriner, 14(4), 408–416.
Kurnia, R., Abdusysyahid, S., & Fitriyana, F. (2023). Strategi pengembangan kelompok usaha pembudidaya ikan nila (Oreochromis niloticus) Mina Kolam Mandiri Jaya di Desa Ponoragan Kecamatan Loa Kulu. Jurnal Perikanan, 13(3), 902–913. https://doi.org/10.31258/jp.v13i3.612
Rahmad, R., Sumiati, R., Nurdin, E., & Wibowo, R. (2019). Molecular detection of Tilapia Lake Virus (TiLV) in farmed tilapia in Indonesia. Aquaculture Reports, 15, 100221. https://doi.org/10.1016/j.aqrep.2019.100221
Srivorakul, S., Lertwanakarn, T., Wattanaporn, K., Phuthaworn, C., & Surachetpong, W. (2025). Development of RT-RPA assay for TiLV detection. Infectious Diseases Journal, 10(1), 45–52. https://doi.org/10.3390/idj10010045
Surachetpong, W., Roy, S. R. K., & Nicholson, P. (2020). Tilapia Lake Virus: The story so far. Fish Pathology, 55(4), 425–438. https://doi.org/10.3147/jsfp.55.425
Taukhid, T., Sari, P., Hidayat, F., Yulianti, D., & Kurniawan, R. (2025). Resistance of Indonesian tilapia strains (Oreochromis niloticus) to Tilapia Lake Virus disease (TiLVD). Bulletin of the European Association of Fish Pathologists, 45(2), 123–132.
World Organisation for Animal Health. (2021). Tilapia Lake Virus disease (TiLVD): Manual of diagnostic tests for aquatic animals. https://www.woah.org/fileadmin/Home/eng/Health_standards/aahm/current/TiLVD.pdf
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