Perbandingan Proses Clearing Antara Xylol dan Minyak Gandapura Terhadap Kualitas Preparat Awetan Kutu Tikus ( Xenopsylla Cheopis )
DOI:
https://doi.org/10.62383/quwell.v1i4.940Keywords:
Xylol, Coconut Oil, Cleansing, Preparation QualiAbstract
Xylol is a dangerous and toxic substance. The use of Xylol in the Anatomical Pathology laboratory is considered common for the stage of “cleansing” both for the process of tissue entry and sanation. Xylol is used in Anatomical Pathology laboratories because it is soluble with dehydrating agents and can disintegrate paraffin as a drying agent. Xylol is part of Gandapura oil hydrocarbons. Research aimed at finding alternative substitutes for xilol was conducted using Gandapura oil using experimental methods. Samples using Mouse Pins (Xenopsylla cheopis) can show thinning of the skin layer with connective tissue due to the use of “cleansing” agents.with Xylol 15 and 30 minutes Coconut oil 15 and 30 minutes. The parameters used are a description of the color quality of the preparation, the clarity of the preparation and the thoroughness of the preparation. The results of the study showed A1 preparations with a result of 3 bright,3 moderately bright, 2 clear, 4 MImoderately clear and 6 hours. A2 preparation with results of 2 bright, 4 moderately bright 3 clear, 3 moderately clear, and 6 minutes. Preparation B1 with a result of 4 bright 2 well-lit, 5 rows 1 fairly clear and 6 hours. B2 preparation with a result of 1 bright, 5 moderately bright, 2 clear 4 fairly clear 5 grains and 1 out of order. The use of coconut oil decrypted gives a good quality to the preparation compared to using Xylol, but it cannot be used for a long time.
References
Abdullah. (2020). Program studi DIV analis kesehatan fakultas ilmu keperawatan dan kesehatan Universitas Muhammadiyah Semarang 2020, p. 16066.
Firmansyah, D., & Saepuloh, D. (2022). Daya saing: Literasi digital dan transformasi digital. Journal of Finance and Business Digital, 1(3), 237–250.
Fitriani, F., Husmimi, H., & Akmal, M. (2018). Aplikasi metode emersi fiksatif berbeda terhadap morfologi histologi testis dan epididimis kambing lokal (Capra sp.). Jurnal Agripet, 18(1), 24–29.
Hernandes, M. Z., et al. (2017). Halogen atoms in the modern medicinal chemistry: Hints for the drug design. Current Drug Targets, 11(3), 303–314.
Iswara, A., & Fitri, N. (2017). Pengaruh variasi waktu clearing terhadap kualitas sediaan awetan permanen Ctenocephalides felis. 1(1), 12–15.
Khristian, E. (2018). Potensi minyak gandapura sebagai pengganti xilol dalam pembuatan sediaan mikroskopis otak mencit. Prosiding Pertemuan Ilmiah Nasional Penelitian & Pengabdian Masyarakat (Pinlitamas 1), 1(1), 638–644.
Kuniarti, I., Sumanto, D., & Alhamidy, F. (2007). Strengthly of permanent preparation of Culex pipiens eksperimen. Sampel berupa larva. Litbang Universitas Muhammadiyah Semarang, 50–55.
Kurniawati, I., Sumanto, D., & Alhamidy, F. (2007). Daya tahan sediaan permanen larva Culex pipiens dengan perlakuan dehidrasi menggunakan konsentrasi alkohol yang berbeda. Jurnal Litbang, 3(2).
Kusumo, P. (2015). Proses inaktivasi enzim gaultherase melalui mixed-drying extraction untuk pengambilan gaultherin sebagai antikanker. In Seminar Nasional Teknik Kimia "Kejuangan".
Lael, B. F. (2018). Perbedaan penggunaan xilol (xylene) dan toluol (toluene) pada proses clearing terhadap kualitas preparat awetan permanen Cimex lectularius (Doctoral dissertation, Universitas Muhammadiyah Semarang).
Meri, J., Sih, A., et al. (2016). Rickettsia pada pinjal tikus (Xenopsylla cheopis) di daerah pelabuhan Semarang, Kupang, dan Maumere. Indonesian Bulletin of Health Research, 44(4), 237–244.
Prabasari, N. S., Santosa, B., & Ariyadi, T. (2018). Pengaruh variasi waktu perendaman koh 10% terhadap kualitas sediaan permanen Riphicephalus sanguineus. Skripsi, Fakultas Ilmu Keperawatan dan Kesehatan Universitas Muhammadiyah Semarang.
Priyotomo, Y. C., Santoso, L., Martini, M., & Hestiningsih, R. (2015). Studi kepadatan tikus dan ektoparasit di daerah perimeter dan buffer pelabuhan laut Cilacap. Jurnal Kesehatan Masyarakat, 3(2), 86–95.
Reichenbach, N., et al. (2019). Inhibition of Stat3-mediated astrogliosis ameliorates pathology in an Alzheimer's disease model. EMBO Molecular Medicine, 11(2), e9665.
Retnawati, H. (2016). Analisis kuantitatif instrumen penelitian (Panduan Peneliti, Mahasiswa, dan Psikometrian). Parama Publishing.
Setyaningrum, E., et al. (2020). Identifikasi ektoparasit pada tikus (Rattus sp.) sebagai vektor penyakit pes di areal pelabuhan panjang kota Bandar Lampung. Jurnal Medika Malahayati, 4(2).
Sugiyono, D. (2018). Metode penelitian. Bandung: Penerbit Alfabeta.
Ustiawan, A. (2018). Xenopsylla cheopis. Jurnal Litbang Pengendalian Penyakit Bersumber Binatang Banjarnegara, 4, 57453.
Widiyanti, M., & Putu, N. L. (2018). Pola perindukan nyamuk yang ditangkap di perindukan di Kabupaten Buleleng dan manfaatnya sebagai bahan praktikum dalam perkuliahan zoologi invertebrata. Jurnal Ika, 11(1), 27–41.
Yohana, W. (2017). Perbandingan cairan fiksasi bouin dengan buffer formalin terhadap hepar tikus putih. Journal of Syiah Kuala Dentistry Society, 2(2).
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